Sample Preparation: The sample is prepared by extracting the DNA and adding it to a reaction mix containing primers, probes, and other reagents. Partitioning
Protocols for Digital PCR The following is a typical protocol for digital PCR: Digital PCR- Methods and Protocols
Principles of Digital PCR Digital PCR is based on the principle of separating a sample into many partitions, each containing a single copy of the target DNA sequence. The partitions are then subjected to PCR amplification, and the resulting fluorescence signals are studied to identify the presence or absence of the target sequence in each partition. By counting the number of positive partitions, the concentration of the target sequence in the original sample can be determined. Sample Preparation: The sample is prepared by extracting
Methods of Digital PCR There are several methods of digital PCR, including: Droplet Digital PCR (ddPCR) By counting the number of positive partitions, the
